By A Grothendieck
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5. Density gradient centrifugation studies of acetyl CoA carboxylase at 4% and at 25% (Numa and Ringelmann, rg6~). The solid lines repraent the cvboxylase activity and the dashed lints the fatty acid synthctase activity. is initially active but decays within several minutes, as a result of the transition into the “smau” form. The dissociation and the concomitant loss of enzymic activity could be prevented by the addition of citrate or 40 P. LYNEN isocitrate to the assay mixture. The physiological sigruficance of the stimulatory effect of citrate alone remains obscure.
Biochim. biophys. Ada, 84, 750. KOSHLAND, D. , JR. 1958 . Roc. nutn. A d . Sci. R. ec, 98. KOSHLAND, D. ,JR. 1968. Adv. Enzyme Regulation, 6,291. R. (1@3). Biochem. biophys. Res. Commun.. 13, 267. , and C ~ O P IP . (1963). Biochim. biophys. Acto, 70,627. LOWENSTEIN,J. (1968). Biochem. Soc. , 27,61. LYNEN,F.. , and EGGH. (1964). Biochem. , 340,gs. MASORO. J. (1962). , 3, 149. MONOD. J.. and JACOB, F. Cold S p ' q Harb. Symp. ant. , 26, 389. , WYMAN, J.. and CHANGEUX, J. P. mo ec. , 12, 88. W. M..
Bourgeois: In our bin* experiments we use intact phage DNA of molecular weight 30 million. We have also shown bindmg to I million molecular weight pieces of that DNA, obtained by sonication. We did break the DNA down further using spleen DNase and followed the killing curve of operator (assayed by its bindmg activity) compared with the reduction in molecular weight of the DNA (determined by sedimentation equilibrium). From such a prelunrnary experiment the operator appears to be very small indeed, of the order of 20 base pairs, the only assumption being that the DNase cleaves DNA at random.
A general theory of fibre spaces with structure sheaf by A Grothendieck